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	Provedor de dados PAB (2) Autor Silva,Tatiane Loureiro da (2) Attílio,Lísia Borges (1) Harakava,Ricardo (1) Mendes,Beatriz Madalena Januzzi (1) Miyata,Luzia Yuriko (1) Mourão Filho,Francisco de Assis Alves (1) Scherwinski-Pereira,Jonny Everson (1) Stipp,Liliane Cristina Libório (1) Mais... Palavra-chave Antimicrobial peptide (1) Citrus sinensis (1) Ex situ conservation (1) Germplasm bank (1) Micropropagation (1) Tissue‑specific promoter (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2011 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter PAB Attílio,Lísia Borges; Mourão Filho,Francisco de Assis Alves; Harakava,Ricardo; Silva,Tatiane Loureiro da; Miyata,Luzia Yuriko; Stipp,Liliane Cristina Libório; Mendes,Beatriz Madalena Januzzi. The objective of this work was to produce transgenic 'Pêra' and 'Valência' sweet orange plants using the D4E1 gene driven by the Arabidopsis thaliana phloem protein (AtPP2) promoter and to quantify transgene expression in different transformation events. Genetic transformation experiments were carried out with epicotyl segments co‑cultivated with Agrobacterium tumefaciens. Six plants from 'Pêra' sweet orange and seven plants from 'Valência' sweet orange were confirmed as different transgenic events by means of the polymerase chain reaction (PCR) and the Southern blot techniques. Transgene expression was quantified using real‑time quantitative PCR. D4E1 gene expression levels vary from 5 up to 50 times among different transformation events. Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Antimicrobial peptide; Tissue‑specific promoter. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000700006 In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions PAB Silva,Tatiane Loureiro da; Scherwinski-Pereira,Jonny Everson. The objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol - at 1, 2 and 3%) and abiscisic acid - ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L-1). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20ºC was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20ºC on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ex situ conservation; Germplasm bank; Micropropagation. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000400007 Registros recuperados: 2 Primeira ... 1 ... Última

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